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Development Biology Methods and Protocols for Mouse Embryo
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Development Biology Methods and Protocols for Mouse Embryo
小鼠发育生物学与胚胎实验方法
Language:  Chinese,Latin name
Author:  Jin Yan
Pub. Date:  2005-01 Weight:   kg ISBN:  9787117065290
Format:  Hardcover Pages:  587Pages
Subject:  Zoology > Mammals
Series:   Size:  185*260mm
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全书分2篇共25章,第一篇包括9章内容,重点介绍发育生物学的相关概念,一般过程和发育的基本规律,小鼠的胚胎发育概况,尤其是发育生物学的基本问题。
第二篇包括16章,详细介绍了小鼠胚胎实验多种的技术和方法,包括小鼠的体外受精及胚胎移植的实验操作,基因转染技术,着重介绍了如何制备转基因小鼠,基因敲除小鼠,嵌和体小鼠,以及如何系统的对这些小鼠和实验结果进行分析的方法,包括报告基因,细胞标记,核型分析与染色体整合基因定位等。本片还介绍了小鼠基因组研究与单细胞消减杂交,受精卵与胚胎的冻存处理与胚胎性别鉴定,胚胎标本的制作及基因和基因产物的检测,牙胚发育与腭裂动物模型的建立与分析,此外还介绍了各阶段鼠胚的体外分离与培养,组织重组与器官培养等都进行了较深入的阐述。




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