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This collected works contains following 30 papers published in Chinese journals in recent years. It may be used as research materials in the field.
1、Supplemental study on the pollen morphology of the tribe Hyoscyameae (Solanaceae) and its systematic significance (In English)
2、Pollen morphology of the genus Lasianthus (Rubiaceae) and related taxa from Asia (In English)
3、STUDIES ON POLLEN MORPHOLOGY OF GENUS DIDYMOCARPUS (GESNERIACEAE) IN CHINA (In English)
4、Pollen Morphologies of Typhonium Schott and Its Genetically Close Genera (Araceae) (In English)
5、A STUDY ON POLLEN MORPHOLOGY OF TUNDRA PLANTS FROM BARROW, ARCTIC (In English)
6、POLLEN MORPHOLOGY AND DETAILED STRUCTURE OF THE GENUS NANNOGLOTTIS AND ITS SYSTEMATIC IMPLICATIONS (In English)
7、Pollen Morphology of Bignoniaceae in China (In English)
8、Pollen Morphology of Aceraceae and Its Systematic Implication (In Chinese with English Summary)
9、Study of the pollen morpholgic appraisable point of pinaceaeous main genera and their ecological environment (In Chinese with English Summary)
10、POLLEN MORPHOLOGY OF 6 SPECIES FROM SONNERATIA AS A FOSSIL GUIDE (In Chinese with English Summary)
11、Microtubule Organization in Germinated Pollen of Pinus thunbergii (In English)
12、Survey of airborne pollen and pollen hypersensitivity in Wuhan. (In Chinese with English Summary)
13、Pollen germination in vitro of Mei flower (In Chinese with English Summary)
14、Pollen germination in vitro of chrysanthemum cultivars with small inflorescences and several species of Dendranthema (In Chinese with English Summary)
15、Microfilament Skeleton in the Pollen and Pollen Tube of Cunninghamia lanceolata (In Chinese with English Summary)
16、Research on Haifengteng Pollen Separation and Morphology (In Chinese with English Summary)
17、Study on the Pollen Storage and Viability of Lagerstroemia indica (In Chinese with English Summary)
18、STUDIES ON THE POLLEN MORPHOLOGY AND POLLEN GERMINATION MORPHOLOGY IN GRAPE CULTIVARS (In Chinese with English Summary)
19、Study on the Pollen Morphology and Pollen Development of Liliaceae (In Chinese with English Summary)
20、Study of Classification of Different Chinese Yam(Dioscorea oppositaThunb.) by Pollen Morphology (In Chinese with English Summary)
21、Pollen Morphology of Some Chinese Traditional Roses (In Chinese with English Summary)
22、Pollen Morphology of Twelve Species in Brassicaceae (In Chinese with English Summary)
23、STUDY ON POLLEN MORPHOLOGY AND ITS GEOHISTORIC SIGNIFICANCE OF TWO ENDANGERED ELMS PECULIAR TO MT. LANGYA,CHINA (In Chinese with English Summary)
24、Pollen Morphology of 10 Species of Ipomoea by Scanning Electron Microscope(SEM) (In Chinese with English Summary)
25、Comparative Analysis of Pollen Germination Between Different Black Locusts (In Chinese with English Summary)
26、Classification of Wintersweet Cultivars by Pollen Morphology (In Chinese with English Summary)
27、Observation of 5 Lagerstroemia indica Cultivars Pollen Germinationby Means of Fluorescence Microscope (In Chinese with English Summary)
28、TESTS OF POLLEN QUALITY AND DEVELOPMENT OF POLLEN TUBES IN LIRIDENDRON (MAGNOLIACEAE) (In Chinese with English Summary)
29、ULTRASTRUCTURE OF MICROFILAMENTS IN POLLEN AND POLLEN TUBES OF HOSTA VENTRICOSA(=H.COERULEA) (In Chinese with English Summary)
30、MEIOSIS OF POLLEN MOTHER CELLS AND DEVELOPMENT OF POLLEN IN CHINESE SACRED LILY (NARCISSUS TAZETTA VAR. CHINENSIS ROEM. (In Chinese with English Summary)

1、Supplemental study on the pollen morphology of the tribe Hyoscyameae (Solanaceae) and its systematic significance (In English)
天仙子族(茄科)的花粉形态补充研究及其系统学意义(英文)
[Author]: ZHANG Zhi-Yun1,YANG Dong-Zhi2,JOONGKU Lee3,LI Liang-Qian1(1.Herbarium(PE)& State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China;2.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China;3.Korea Research Institute of Bioscience and Biotechnology,Daejeon 305-806,Korea)
[Abstract]: Pollen grains of 23 species in seven genera of the tribe Hyoscyameae(Solanaceae)and three related genera(Atropa,Mandragora,Lycium)were investigated under SEM.The pollen grains are globose,subglobose,oblate or prolate in shape.Germination apertures were absent or present and are irregularly poroid,3-colpate,4-colpate,3-or 4-colpate,pantocolpate or 3-colporate.The exine ornamentation was spinulose,tuberculate,variously striate,striate-foveolate,striate-reticulate,rugose-sinuate,minutely reticulate or reticulate.The colpal membrane was nearly smooth but with granular,spinulose or tuberculate protuberances.The pollen grains show great variation between genera in the Hyoscyameae in the presence and type of a germination aperture and exine ornamentation.These may be important characters in revealing phylogenetic relationship in the tribe.The evolutionary trend in germination apertures ranges from absent or presence to 3-or 4-colpate then to 3-colporate.Anisodus,which lacks germination apertures,is considered to be primitive,while genera with 3-colporate pollen grains,such as Przewalskia and Hyoscyamus are believed to be advanced.
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2、Pollen morphology of the genus Lasianthus (Rubiaceae) and related taxa from Asia (In English)
亚洲茜草科粗叶木属及其相关属植物花粉形态研究(英文)
[Author]: 1,2Min CAI 1Hua ZHU 3Hong WANG 1(Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China) 2(Graduate University of Chinese Academy of Sciences, Beijing 100049, China) 3(Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China)
[Abstract]: Pollen morphology of 16 Asian species including two subspecies and one variety of the genus Lasianthus and five species from five related genera was examined by light microscopy and scanning electron microscopy. Lasianthus Jack is eurypalynous. The pollen grains are small to medium in size and spheroidal or subprolate in shape, with a few being prolate. Pollen morphology is remarkably diverse, particularly in aperture and exine ornamentation. The apertures are usually (3–)4–(–5)-zonoaperturate, being porate and pororate. The number of apertures varies from three to five, with the majority of species being 3–4 zonoaperturate. The exine is usually perforate, finely reticulate or coarsely reticulate. Pollen data appear to be morphologically informative and useful for distinguishing among species of Lasianthus, and for elucidating the relationships among some species.
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3、STUDIES ON POLLEN MORPHOLOGY OF GENUS DIDYMOCARPUS (GESNERIACEAE) IN CHINA (In English)
中国苦苣苔科长蒴苣苔属花粉形态研究(英文)
[Author]: YAN Zhi-jian~1, LI Zhen-yu~2 rovince and the Chinese Academy of Sciences, Guiyang 550002, China; 2. Institute of Botany, Academia Sinica, Beijing,100093, China
[Abstract]: Morphological studies of pollen of eight species of genus Didymocarpus (Gesneriaceae) in China are reported in this paper. The pollen grains of genus Didymocarpus are tricolporoidate, subprolate in shape, lobate-circular in polar view, elliptical in equatorial view. Colpi are rather long and rather narrow, colpus membrane with granular processes and constricted in the middle. The study shows that pollen of species in genus Didymocarpus are similar in shape and colpus characteristics, but different in ornamentations.
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4、Pollen Morphologies of Typhonium Schott and Its Genetically Close Genera (Araceae) (In English)
犁头尖属及其近缘属植物天南星科的花粉形态(英文)
[Author]: LI Ai-hua,SHEN Yun-guang,GUAN Kai-yun,WANG Zhong-lang(Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China)
[Abstract]: The pollens of 18 plants of Typhonium Schott and its genetically close genera, 8 first reported plants and eleven endemic plants in China were studied with a scanning electron microscope. The results show that the plants of four genera of Tribe Areae have basically similar pollens,which are spherical,in-aperturate,intectate and spinulose,but they greatly differed in pollen size,spinule length,shape and density,basal spinule diameter,and inter-spinule ornamentation,and this can be used as one of the species-identifying characteristics. The pollen morphologies support the viewpoint that Tribe Areae is the most evolutive taxon of Araceae. Granular exine sculpture is the most remarkable pollen features of Pinellia Tenore plants. The pollen morphologies do not support the viewpoint that T. calcicolum C. Y. Wu ex H. Li et al. and T. kunmingense H. Li should be merged. T. omeiense H. Li has very small pollens and as results is thought a more evolutive species. The species of T. omeiense H. Li differ in pollen morphology,even more than the genera do,and hence the classification of this genus may need to be modified to a large extent, which agrees with the results achieved by Susanne with cpDNA bands.
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5、A STUDY ON POLLEN MORPHOLOGY OF TUNDRA PLANTS FROM BARROW, ARCTIC (In English)
北极Barrow地区几种冻原植物的花粉形态(英文)
[Author]: ZHANG Xiao-ping (College of Life Science, Anhui Normal University, Wuhu 241000) (State Key Laboratory of Paleobiology and Stratigraphy, CAS, Nanjing 210008) TANG Ling-yu (The Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000) (Nanjing Institute of Geology and Paleontology, The Chinese Academy of Sciences, Nanjing 210008) ZHOU Zhong-ze (College of Life Science,Anhui University, Hefei 23000) ZHANG Qing-song (Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101)
[Abstract]: Pollen morphology of 12 species of the tundra plants from Barrow, Arctic are investigated in the present study. The result shows that the pollen grains are mainly spheroidal, subspheroidal and oblate, cocoon-formed or tetrads. The types of aperture are mostly tricolporate or tricolpate, rarely pantoporate. They are radially or bilaterally symmetric. All these plants from which the pollen meterials were collected for this study are found to occur commonly in Arctic area, and belong to 12 species in 10 families. Most of them, except for 3 species which are bushes, are perennial or annual herbs. Investigation on pollen grains of tundra plants can provide significant data for comparative study of fossil pollen and for reconstruction of paleovegetation and paleoclimate in the Barrow area.
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6、POLLEN MORPHOLOGY AND DETAILED STRUCTURE OF THE GENUS NANNOGLOTTIS AND ITS SYSTEMATIC IMPLICATIONS (In English)
毛冠菊属的花粉形态和结构及其系统学意义(英文)
[Author]: Zhang Xiao ping (Department of Biology , Anhui Normal University, Wuhu 241000)
[Abstract]: This study presents a detailed survey and discussion on the pollen morphology and structure of Nannoglottis with light microscopy(LM), SEM, and TEM. Measurements of the polar axis and equatorial diameter, exine thickness, spine length, and spine number at each lobe are made. Differences were observed both between representatives of different tribes and between species of the same genus. The principal differences in pollen among the various species were in size, shape and subtle variations of the surface ornamentation. Three kinds of pollen types can be recognized in the whole genus, namely, spheroidal type, prolate type and oblate type. Combined with both palynological and biosystematic evidences, the data support Hand Mazzetti’s taxonomic treatment that put Nannoglottis in the tribe Astereae, rather than under the tribes Senecioneae or Inuleae.
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7、Pollen Morphology of Bignoniaceae in China (In English)
中国紫葳科花粉形态的研究(英文)
[Author]: WEI Zhong-Xin, WANG Hong, JIN Qiao-Jun (Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China)
[Abstract]: The prains of 27 native and introduced species belonging to 18 genera of Bignoniaceae were examined by light and scanning electron microscopes.Some of which were reported or examined by scanning electron microscope for the first time.Though,the Bignoniaceae are conspicuously eurypalynous, their apertures can be divided into 3 (-4) colpate, 3 (-4) colporate, 3- porate, stephanocolpate and pantocolpate types. The exine sculptures include foveo- late, rugulate, reticulate (fine and coarse reticulations) and spinulate, or psilate. Most pollen exist as monads but a few are tetrad. In the genus of Radermachera, the pollen aperture and sculpture are different between R. sinica and R. yunnanensis. The pollen of R. sinica is3-porate and reticulate sculpture, but that of R. yunnanensis is 3- colpate and foveolate sculpture. In the family of Bignonaceae, the pollen morphology is valuable both in generic delimi- tation and in the study of systematic relationships, especially aperture and the exine sculpture..
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8、Pollen Morphology of Aceraceae and Its Systematic Implication (In Chinese with English Summary)
槭树科花粉形态及其系统学意义
[Author]: TIAN Xin, JIN Qiao-Jun, LI De-Zhu, WEI Zhong-Xin, XU Ting-Zhi (Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204,China)
[Abstract]: Pollen gains of 33 species in 21 sections of Acer,together with two species of Dipteronia were observed by light microscope(LM) and scanning electronic microscope (SEM). The shape of pollen grains in this family varies from subspheroidal to prolate in equatorial view and is 3-lobed circular in the poilar view. The aperture types of both species in Dipteronia are tricolporate. This type can also be found in 4 sections of Acer, but most species in Acer are tricolpate. As for the pollen exine sculpture, both species in Dipteronia and most spcies in Acer are striate, but two sections in Acer are finely striate-reticulatoid or regulate. By the studies of pollen morphology and other evidence, it is inferred that :(1)Sect.Palmata, Sect.Spicata and Sect.Microcarpa may be the closest groups in Acer to Dipteronia; (2) A. distalum and A. nipponicum represent two related monotypic sections; (3) The pollen of A. pseudoplatanus, which is different from other four species in Sect. Acer,is similar to that of A. saccharum. It therefore indicates that these two species have a close relationship; (4) Sect. Carpinifolia ,which has finely striate-reticulatoid exine sculpture, and Sect. Negundo, which has regulate exine sculpture, may be the most specialized sections in Acer; (5) Variation from tricolporate to tricolpate is regarded as an evolutionary trend in Aceraceae. The genus Dipteronia, therefore,is more primitive than the genus Acer.
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9、Study of the pollen morpholgic appraisable point of pinaceaeous main genera and their ecological environment (In Chinese with English Summary)
松科花粉鉴定要点及其生态环境特征研究
[Author]: SUN Ai-zhi MA Yu-zhen HUANG Chang-qing Wu Hui-ning(College of Resources and Environmental Science,Lanzhou University,Lanzhou 730000,China)
[Abstract]: This paper is a detailed study on the pollen morphologic description,appraisable point and comparison between five genera (Tsuga,Abies,Cedrus,Picea,Pinus) and two subgenera (Diploxylon type, Haploxylon type) of Pinaceae.Five genera have been distinguished by the shape and lumina of saccus, cap’s texture,transitional characteristics between body and saccus and Diploxylon and Haploxylon types distinguished from two appraisable points.Moreover,their favorable environment has been studied.For almost Pinaceae,the suitable annual average temperature is different.Haploxylon type may be suitable for low temperature or dry and cold environment,whose average altitude may be no more than 1200 m;however the Diploxylon type may have adapted itself to warm and humid conditions,whose altitude may exceed 1200 m.
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10、POLLEN MORPHOLOGY OF 6 SPECIES FROM SONNERATIA AS A FOSSIL GUIDE (In Chinese with English Summary)
海桑属6种植物花粉形态兼化石花粉指南
[Author]: MAO Li-mi 1) ,LI Ni-ya 2) ,WANG Dong 2) ,CHEN Jian-hai 3) and ZHONG Cai-rong 3) 1) State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,CAS,Nanjing 210008 2) College of Life Sciences,Central China Normal University,Wuhan 430079;3) Administration Bureau of Dongzhai National Mangrove Reserve,Haikou 571129
[Abstract]: The distribution of Sonneratia extends from East Africa to Indo-Malesia,northern Australia,New Guinea and the Western Pacific,and the Sonneratia vegetation comprises the main communities of coastal mangrove forests. This paper presents pollen morphological results based on LM and SEM observations for six species of the genus. Pollen wall debris obtained by the MP FastPrep120 were observed using SEM in order to examine the exine stratification. Four pollen types of the Sonneratia species can be grouped,namely A type(Sonneratia alba,S. × hainanensis,S. × gulngai),B type(S. ovata),C type(S. caseolaris) and D type (S. apetala). Pollen keys are established based on their morphological characteristics. Pollen morphological study on extant Sonneratia contributes our understanding of the evolution of mangrove paleobotany and paleoecosytem changes,which expands the mangrove paleoecological study field. Pollen morphological investigations of the extant Sonneratia plants provide useful information,which are important keys to the identification of fossil pollen grains of the same genus or related taxa. The significance of extant Sonneratia pollen on fossil pollen identification and palecological study is also discussed.
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11、Microtubule Organization in Germinated Pollen of Pinus thunbergii (In English)
黑松花粉与花粉管中的微管分布(英文)
[Author]: Li Guoping~ 1,2 Huang Qunce~1 Yang Lusheng~2 Qin Guangyong~1(1. Provincial Key Laboratory of Ion Beam Bio_Engineering, Zhengzhou University Zhengzhou 450052;2.Department of Environment and Life Sciences, Putian University Putian 351100)
[Abstract]: Gymnosperms originated earlier than angiosperms. Elongation in gymnosperms pollen tube exhibits some evolutionarily primitive characteristics, i.e. slow germination and growth, branching and longevity. These differences may be orchestrated by cytoskeletal organization. In the study, microtubule organization in germinated pollen of Pinus thunbergii is investigated using a confocal laser scanning microscope after immunofluorescence labeling. Within germinated pollen, microtubules are arranged obliquely or transversely. In elongated tubes, microtubules are arranged along the longitudinal axis. An enriched microtubules network is presented in the pollen tube tip and has a unique functional role. Following colchicine treatment, the organization of microtubules within pollen tubes is disrupted and the morphology of pollen tubes is changed. Colchicine significantly induces tube tip swelling. Swollen tip is considered to be caused by the disorganization of microtubules in the tip. Our results indicated that microtubules in gymnosperms pollen tube tip play an important role in maintaining tip integrity. The present paper also suggests that microtubules participate in directing tip extension in gymnosperms pollen tube and gymnosperm pollen tubes elongate in a mechanism that is different from angiosperm pollen tubes.
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12、Survey of airborne pollen and pollen hypersensitivity in Wuhan. (In Chinese with English Summary)
武汉市气传花粉与花粉过敏调查
[Author]: Li Wenjing. Department of Allergy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030
[Abstract]: Objective To survey the species, amount of the airborne pollen in Wuhan city, and the pollen hypersensitivity, and their correlations. Methods By Durham gravity method, samples of atmospheric particles were collected in Wuhan from Nov. 2003 to Oct. 2004. The species and number of the pollen were statistically analyzed. Results 21610 grains of pollen were collected. The peak of pollen occurred in March or September, and Platanus had the largest amount. The most common allergen was spring pollen. ...
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13、Pollen germination in vitro of Mei flower (In Chinese with English Summary)
梅花花粉离体萌发和花粉管生长研究
[Author]: ZHAO Hong-Bo,FANG Wei-Min,CHEN Fa-Di(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
[Abstract]: The pollen germination in vitro of four cultivars of Prunus mume were detected.The results showed that the optimum culture medium of pollen germination of P.mume was ME3+200 g•L-1PEG4000(pH5.0),at which the pollen germination ratio of cultivars ’Dan Fenghou’,’Jiuguan Lü’e’,’Xuanyan Gongfen’,’Yueguang Yudie’reached 58.1%,45.3%,84.5%,50.7% respectively.PEG4000 can remarkably accelerate pollen germination of P.mume,and the optimal concentration was 200 g•L-1.The effects of the sucrose on pollen germination was not prominent,only low concentration of the sucrose(50 g•L-1)can accelerate pollen germination of some cultivars(e.g.’Jiuguan Lü’e’),however,high concentration of sucrose would inhibit strongly pollen germination and the growth of pollen tubes.There was no significant difference between the effects of solid and liquid cultures on pollen germination in vitro.
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14、Pollen germination in vitro of chrysanthemum cultivars with small inflorescences and several species of Dendranthema (In Chinese with English Summary)
栽培小菊和几种菊属植物花粉离体萌发研究
[Author]: ZHAO Hong-bo,CHEN Fa-di*,FANG Wei-min (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
[Abstract]: The pollen germination capacity and growth of pollen tubes of Dendranthema indicum Des Moul.,D.vestitum Hemsl.and three cultivars of chrysanthemum(43-2,46-1,48-2)with small inflorescences were detected to obtain the optimal culture media,culturing methods and conditions of pollen germination in vitro of chrysanthemum(Dendranthema).The results showed that the optimal culture medium of pollen germination of D.indicum and D.vestitum was ME3+ 300 g•L -1 PEG1500,in which the pollen germination capacity reached to 54.1% and 46.3% respectively;and the optimal culture media of 46-1,48-2 were ME3+ 300 g•L -1 PEG1500 and ME3+ 200 g•L -1 PEG4000(the significance between them was indifferent),in which the greatest germination capacity reached to 25.3% and 29.3%.The effects of H3BO3 and Ca 2+ on pollen germination capacity were not prominent,but they could improve the growth of pollen tubes,pollen tubes were more straight,thick,and suitable in length.And the effect of sucrose on pollen germination was not prominent too.The significance between solid and liquid culturing on pollen germination in vitro was indifferent.The same as the significance of light and dark disposals.With temperature increase the pollen germination capacity of D.indicum,D.vestitum and chrysanthemum cultivars 43-2,48-2 were all increasing,while the increased extent of the latter was greater.
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15、Microfilament Skeleton in the Pollen and Pollen Tube of Cunninghamia lanceolata (In Chinese with English Summary)
杉木花粉和花粉管中微丝骨架
[Author]: Gan Guofeng Xu Jin Lu Ye Shi Jisen (Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037)
[Abstract]: Microfilament skeleton plays important role during the process of pollen development and pollen tube growth.We observed the distribution of microfilaments in the pollen and pollen tube of Cunninghamia lanceolata by phalloidin labeling fluorescence and confocal laser scanning microscope.The images showed different distribution of microfilaments during pollen germination process.At the early stage of pollen germination,filiform microfilaments occurred in the pollen cytoplasm,and formed thick F-actin bundles around the nuclear surface;With pollen tube growth,microfilament bundles extended and distributed axially in the pollen tube;Thick F-actin bundles were absent near the pollen tube tip and a dense actin cap was formed with thin microfilament fragments in the tip of pollen tube.The microfilament density in the pollen and pollen tube was different from other angiosperms.The results sugguested that the difference in the microfilament density might be related to the slow growth of a pollen tube in C.lanceolata.
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16、Research on Haifengteng Pollen Separation and Morphology (In Chinese with English Summary)
海枫藤花粉块壁剥离与花粉形态探究
[Author]: GUO Qing,WANG De-bin,ZHAI Shu-hua,CHEN Zi-niu(Department of Biology,Kunming Teachers College,Yunnan Kunming 650031,China)
[Abstract]: The effects of different methods for separation the Haifengteng pollen had been studied.20%KOH and 10min resulted in good effect for separation the Haifengteng pollen.The pollen grains of Haifengteng have different shapes.
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17、Study on the Pollen Storage and Viability of Lagerstroemia indica (In Chinese with English Summary)
紫薇花粉贮藏及花粉活力的分析
[Author]: ZHANG Zhao-kun,FENG Zhen,DAI Qing-min,LI Yan-yan,ZHANG Bao College of Forestry,Shandong Agriculture University,271018,Taian,Shangdong,China
[Abstract]: The pollen viability and storage temperature of three cultivars of Lagerstroemia indica were studied in this experiment.The results showed that the pollen viability of three cultivars of Lagerstroemia indica was significantly different.The pollen viabilities of three cultivars could be kept for 164.278 h and for 141.278 h at 4℃ and at-20℃,respectively.However,at the room temperature,the pollen viabilities only could be kept for 18.444 h.
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18、STUDIES ON THE POLLEN MORPHOLOGY AND POLLEN GERMINATION MORPHOLOGY IN GRAPE CULTIVARS (In Chinese with English Summary)
葡萄品种花粉和花粉萌发形态的研究
[Author]: Liu Changen Zhu Lin ( Shandong Vine and Wine-making Institute )
[Abstract]: This paper deals with the pollen morphology and pollen germination morphology in grape cultivars, which were carried out by means of sca-nning electron microscope. The difference of the pollen microshapes am-ong different cultivars-i, e, the differences of the shapes and size of the pollen grains and the differences of the ornamentation and apertures on pollen exines were investigated. These results provide a new method to help identify the grape cultivars. The morphological differences of the pollen germination in liquid culture and on stigma show clearly the effect on grape pollen germination caused by sugar nutrition and water.
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19、Study on the Pollen Morphology and Pollen Development of Liliaceae (In Chinese with English Summary)
百合科（Liliaceae）植物花粉形态及花粉发育的研究
[Author]: Su Lijuan
[Abstract]: Liliaceae is one of the most important plant species in the angiosperm. To research the germ-plasm resources of Liliaceae is regarded as very valuable at home and abroad. Palynology and reproductive biology of plant are the common methods to research the phylogenetic development of plant.Pollen grains of 8 species of Hosta longipes (Franch.et Savat.) Matsum var., Hosta ventricosa (Salisb.) Stearn, Hosta ensata F. Maekawa, Chlorophytum capense (L.)Kuntze, Hemerocallis citrina Baroni, Fritillaria maximowiczii Freyn, Lilium pumilum DC. and Paris verticillata M.-Bieb. were observed and compared by light microscope (LM).Be based on the result of the studies, some conclusions are drawn as follows: The pollen grains of Liliaceae are generally monocolpate (distal). Generally the side of the grain opposite the colpus is narrow, and the colpus generally extends fully to the end of the grain. The equatorial view of the pollen grains are boat-shaped, elliptic or round, and the polar view are elliptic or round. The sculptures are thin- reticulate, thick- reticulate, granulate- reticulate or foveolate- reticulate.The pollen development of H. longipes (Franch. et Savat.) Matsum and its variation, C. capense (L.) Kuntze and P. verticillata M.-Bieb. were studied by paraffin section and light microscope. This paper briefly describes the progress in microsporogenesis,male-gametogenesis, the type of mature pollen, anther development and tapetum development during the pollen development.The results of reproductive biology were observed as follows: The microsporogenesis of Liliaceae is successive type. The tetrads are mostly isobilateral or tetragonal, and less linear. The pace of pollen development in one anther is basically synchronization, but not going all the way. The mature pollen of Liliaceae is 2-cell or 3-cell type. One species has only one type of pollen, but one genus may have two types. There are usually some abnormal pollen in the anther, but the types and odds are different. The tapetum of Liliaceae is secretory tapetum. The mature anther wall is usually consisted by a layer epidermis cell and a layer endothecium cell, except that some species have two layers endothecium cell. Calcium oxalate crystal, which is raphides, is ubiquitous in the anther of Liliaceae. But the amount, phase and position of distribution are
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20、Study of Classification of Different Chinese Yam(Dioscorea oppositaThunb.) by Pollen Morphology (In Chinese with English Summary)
10种长山药花粉形态及分类研究
[Author]: CHEN Zhan-yong1,HUO Xiu-wen1,YIN Chun2,YANG Ming1(1.Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Inner Mongolia Agricultural University,Huhhot 010018,China;2.Vocational and Technical College of Inner Mongolia Agricultural University,Baotou 014109,China)
[Abstract]: Pollen grains of 10 Chinese yam(Dioscorea opposita Thunb.)were observed by scanning electron microscope(SEM).The results showed that the pollen shape of 10 Chinese yam(Dioscorea opposita Thunb.)is spheroidicity with both flat ends and 2 anasulcates.The polar axis length is 11.51 μm(10.02-13.90 μm)and the equatorial axis length is 18.53 μm(16.21-21.05 μm).It is a small pollen(10-25 μm).There were some significant differences on pollen of 10 Chinese yam(Dioscorea opposita Thunb.)in the long polar axis,equatorial axis and P/E ratio respectively.Exine ornamentation is reticulate,foveolate-reticulate,cavernous categories.The result of Q-cluster analysis based on pollen morphology and characteristic indicated that 10 Chinese yam could be classified into three groups when genetic distance was 7.75.The groupⅠincluded Bikeqi Yam and Taoqiang Yam with their small pollen grains,nearly round equatorial view and cavernous exine sculpture.The groupⅡincluded Jiaxiang Yam,Taihuai Yam,Houma Yam,Huilou red peel Yam,Sunfuji Yam and Baoding Yam with medium pollen grain.Taihuai Yam exine sculpture was foveolate-reticulate ornamentation and the other matereils were all reticulate ornamentation.The groupⅢincluded Huaxian Yam and Japan Yam-2 with larger pollen grain and reticulate ornamentation and cavernous ornamentation respectively.
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21、Pollen Morphology of Some Chinese Traditional Roses (In Chinese with English Summary)
部分中国传统月季花粉形态研究
[Author]: BAI Jin-Rong1,2 ZHANG Qi-Xiang1 LUO Le1 PAN Hui-Tang1 YU Chao1(1.College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083;2.Beijing Radiation Center,Beijing 100875)
[Abstract]: The pollen morphology of 15 Chinese traditional roses were examined under scanning electron microscope(SEM).The features of pollen grains were goniotreme,prolate and perprolate,37.59-51.95 μm×17.02-25.33 μm in size.The equatorial view was ellipse,while the polar view was trilobate-circular with tricolporate annularly distributed in equal space.Exine sculpture was striate with apertures.Features of the exine ornamentation showed variability among cultivars.Cluster analysis results indicated that the size and exine sculpture of pollen could reflect the differences among cultivars in a certain extent.According to the evolution of rose pollen,it can be inferred that Roses chinensis ’Parsons’ Pink China’,’Slater’s Crimson China’ and ’Pu Fu Hong’ were more evolutive than other cultivars,while ’Si Mian Jing’ could be regarded as an original cultivar.
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22、Pollen Morphology of Twelve Species in Brassicaceae (In Chinese with English Summary)
12种十字花科植物花粉形态研究
[Author]: REN Hong-yan,Wumaierxiati•Tahan,ZHOU Gui-ling(Key Laboratory of Grassland Resource and Ecology of Xinjiang,College of Pratacultural and Environmental Science,Xinjiang Agricultural University,Urumqi 830052,China)
[Abstract]: Pollen morphology of 12 species belonging to 8 genera of Brassicaceae were observed by using scanning electron microscope(SEM),among which 8 species were reported for the first time.The pollen morphology of 12 species belonging to 8 genera is very similar,their shape varies from prolate to perprolate with reticulate or reticulate-fine sculpture.The pollen grains are most tricolpate(rarely teracolpate).Five pollen types can be distinguished based on the shape of pollen grains,the size of the areole and the number of the aperture:(1)Pollen grains three-colpate,prolate,with reticulate sculpture;(2)Pollen grains three-colpate,perprolate,with reticulate sculpture;(3)Pollen grains three-colpate,prolate,with reticulate-fine sculpture;(4)Pollen grains three-colpate,perprolate,with reticulate-fine sculpture;(5)Pollen grains three-colpate or four-colpate,prolate,with reticulate sculpture.There is not distinct rule about grains morphology on the level of tribe,genus and species.It is not suitable for identification in Brassicaceae only by morphological characteristics of pollen.
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23、STUDY ON POLLEN MORPHOLOGY AND ITS GEOHISTORIC SIGNIFICANCE OF TWO ENDANGERED ELMS PECULIAR TO MT. LANGYA,CHINA (In Chinese with English Summary)
我国两种特有濒危榆树的花粉形态及其地史意义
[Author]: HAN Xue 1,2) ,ZHI Ying-biao 1,3) ,ZHOU Zhong-ze 1,2) ,SUN Zhu-yi 4) YANG Shu-bao 5) ,CHEN Qiao-n 5) and LI Jing-min 1) 1) School of Life Science,Anhui University,Hefei 2300392) Anhui Key Laboratory of Ecological Engineering and Biotechnology,Hefei 2300393) College of Life Science,Nanjing University,Nanjing 2100934) Chuzhou Institute of Foresty Science,Chuzhou 2300005) Laizhou Forestry Experimental Farm of Fujian Province,Laizhou 353004
[Abstract]: Pollen morphology of two endangered elms peculiar to Mt. Langya were examined and described comprehensively under light Microscope (LM). They are Ulmus gaussenii Cheng and U. chenmoui Cheng. The result shows that the pollen grains are mainly subspheroidal in shape and polygonal to rounded or irregular-shaped in polar view. The ornamentation of exine is verrucate,the apertures with 4—6pores are located in equator and subequator U. gaussenii morphology showed typical endangered characteristics when compared with U.pumila. Their geographical distribution,local altitude,annual precipitation and annual accumulated temperature,especially the March climate,are studied to discuss the relationship between the pollen species,morphology,habitat and environment conditions. Therefore,the study will benefit the reconstruction the palaeovegetation,palaeoclimate and paleaoenvironment in Mt. Langya by utilizing the fossil sporo-pollen assemblage.
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24、Pollen Morphology of 10 Species of Ipomoea by Scanning Electron Microscope(SEM) (In Chinese with English Summary)
甘薯属10种植物花粉形态扫描电镜观察
[Author]: CAO Qing-he,ZHANG An,LI Qiang,LI Xiu-ying,WANG Xin,WANG Lu,MA Dai-fu(National Sweetpotato Improvement Centre,Jiangsu Xuzhou Sweetpotato Research Centre,Institute of Sweetpotato Research,CAAS/ The Key Lab of Sweetpotato Genetics and Impromvement,Xuzhou,Jiangsu 221121,China)
[Abstract]: Pollen morphology of 10 Ipomoea species used as breeding materials was examined under scanning electron microscope (SEM),in order to increase the new pollen morphology information and test the reported results.The experimental results show that pollen grains in this genera are uniformly spheroidal in shape and larger in size;most of the pollen apertures are pantoporate,with few inaperturate;3 types of exine sculptures are recognized:echinate,echinate-reticulate and reticulate.Generally,the 10 species plant can be distinguied according to pollen morphology in diameter size,spine length,aperture characters and ornamentation,by detailed SEM observation results.Meanwhile,the pollen morphology of I.wrightii was found to be largely different with the previous report.
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25、Comparative Analysis of Pollen Germination Between Different Black Locusts (In Chinese with English Summary)
刺槐、红花刺槐、四倍体刺槐花粉体外萌发对比
[Author]: Dai Li,Sun Peng,Jiang Jinyu,Hu Ruiyang,Sun Yuhan,Yuan Cunquan,Li Yun(National Engineering Laboratory for Tree Breeding(Beijing Forestry University),Beijing 100083,P.R.China)
[Abstract]: An experiment was conducted to study the effects of sucrose,boric acid and calcium nitrate on pollen germination and pollen tube growth of Robinia pseudoacacia var.decaisneana Carr.,R.pseudoacacia L.,and Tetraploid R.pseudoacacia.Results showed that sucrose,boric acid and calcium nitrate could obviously promote pollen germination and pollen tube elongation,and the influence degrees of the three factors were various between different species of black locusts.The average pollen germination rates of R.pseudoacacia and R.pseudoacacia var.decaisneana were significantly higher than that of Tetraploid R.pseudoacacia.The optimum culture media for pollen germination were different for different species of black locusts.Moreover,the optimum culture media for the germination of pollens collected at different developmental stages were also various for the same species.The optimum culture media for pollen germination were obtained for each species.
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26、Classification of Wintersweet Cultivars by Pollen Morphology (In Chinese with English Summary)
蜡梅品种的花粉形态学分类
[Author]: Wan Huimin1,2 Li Yonghua1 Yang Qiusheng1 (1.College of Forestry,Henan Agricultural University Zhengzhou 450002; 2.Forestry Vocational College, Henan University of Science and Technology Luoyang 471002)
[Abstract]: Pollen morphologies of 33 different wintersweet (Chimonanthus praecox) cultivars in Henan were observed under SEM. Results showed: the wintersweet pollen grains was iso-polar, bilateral symmetry, and existed in the form of single grain, and most of grains were prolate; the pollen had 2-colporate. The exine sculptures were verrucous, vermicule, perforated and cave-shaped; the pollen morphology. The exine sculpture features, pollen shape and pollen size) could be used as the reference bases in the wintersweet cultivars classifications. The pollen morphology was not directly correlated with the external morphology. This study dose not support hypothesis classifying and identifying wintersweet cultivars by pollen morphology alone.
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27、Observation of 5 Lagerstroemia indica Cultivars Pollen Germinationby Means of Fluorescence Microscope (In Chinese with English Summary)
5个紫薇品种花粉萌发的荧光显微观察
[Author]: GU Cui-hua1,WANG Shou-xian2,WANG Min3(1.School of Landscape Architecture,Zhejiang Agriculture and Forestry University,Lin’an 311300,Zhejiang,China;2.School of Information Engineering,Zhejiang Agriculture and Forestry University,Lin’an 311300,Zhejiang,China;3.Park Administration of Fish Viewing of Huagang of Hangzhou,Zhejiang,Hang Zhou 310000,Zhejiang,China)
[Abstract]: To study 5 Lagerstroemia indica Cultivars pollen germination and the course of pollen tube growth were monitored by means of fluorescence microscope.The results showed that within 2~6h after pollination,pollen germinated to form pollen tubes,pollen tube grew from fast to slow;Lagerstroemia floribunda germination of pollen grains were not found at different times after the self-pollination,all pollen grains withered and died,which indicated that Lagerstroemia indica did not accept the homologous pollen,and self-pollination failed.
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28、TESTS OF POLLEN QUALITY AND DEVELOPMENT OF POLLEN TUBES IN LIRIDENDRON (MAGNOLIACEAE) (In Chinese with English Summary)
鹅掌楸属两种植物花粉品质和花粉管生长的研究
[Author]: Zhou Jian;Fan Ruwen(College of Forest Resources & Environment,Nanjing Forestry University Nanjing210037)
[Abstract]: By the methods of fluorochroma(FDA),in vitro,in vivo and nursery stock growing,the pollen quality of the two woody plants, Liridendron chinense and L. tulipifera is examined,and the change in pollen quality was observed during the flowering period.Meanwhile,the pollen germination on the stigma and the growth of pollen tube in the style were investigated under the natural or artificial pollination condition.The results indicate that in vitro condition the pollen germination and pollen tube growth are promoted by the style and ovary tissue obviously.The pollens are affinity,and germinate well on oneself or alien stigma and ovule,however the rate of the pollen tube passing the style is low,only 24%,which is in accordance with our previous study.The results prove again that the developmental phase of the male gametophytes is one of the important factors which influence the seed setting rate,and the style is the region where the inaffinity reaction occurs.
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29、ULTRASTRUCTURE OF MICROFILAMENTS IN POLLEN AND POLLEN TUBES OF HOSTA VENTRICOSA(=H.COERULEA) (In Chinese with English Summary)
紫萼花粉粒和花粉管中微丝的超微结构
[Author]: Hu Shi-yi Li Chun-gui Zhu Cheng (Department of Biology,Peking University,Beijing 100871)
[Abstract]: Ultrastructure of microfilaments in pollen grains and pollen tubes of Hosta ventricosa (=H.coerulea) was investigated.Results indicate that microfilaments with conventional chemi- cal fixation are preserved only in pollen grains,but destroyed in pollen tubes.Microfilaments treated with phalloidin before chemical fixation are found preserved in pollen tubes.In pollen grains a pronounced organization of parallel microfilaments appeared in bundles with its distri- bution characteristics is always restricted to their functional domains where bundles were in close contact with the vegetative nucleus.In young pollen tubes cytoplasmic bundles of micro- filaments appeared also to pass close to the surface of mitochondria,plastids,endoplasmic re- ticulum,vesicles and small vacuoles,and always associated with lipid bodies.These findings strongly indicate that there is a relationship between microfilaments and the movement of vege- tative nucleus and other organelles in the germination of pollen grains and in the growth of pollen tubes.
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30、MEIOSIS OF POLLEN MOTHER CELLS AND DEVELOPMENT OF POLLEN IN CHINESE SACRED LILY (NARCISSUS TAZETTA VAR. CHINENSIS ROEM. (In Chinese with English Summary)
中国水仙花粉母细胞减数分裂和花粉发育的观察
[Author]: Wang lianying Qin Kuijic Li Maoxue,
[Abstract]: 1 . Through smear slides, meiosis of Chinese sacred lily (Narcissus tazetta var. Chinensis Roem. ) is found to be obviously irregular and unbalanced.2. Observations on development of the pellcn grains prove that abortive pollens are formed from microspores with micronuclei, while micro-spores without micronuclci continue to develope into normal functional pollens. The microspores without micronuclei do not have to same chromosome constituent, their development is not synchronous, and no stargh accumulated in them. By artificial germination the pollen shows a rather low percentage (30

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