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No.1 1. Progression and Direction of Humanized Antibody Research 2. The Potential Role of Nuclear Matrix Attachment Regions (MARs) in Regulation of Gene Expression 3. Using Green Fluorescent Protein as a Reporter to Monitor Elimination of Selectable Marker Genes from Transgenic Plants 4. Knockout of the ptsG Gene in Escherichia coli and Cultural Characterization of the Mutants 5. Expression of Goat β-casein Gene Targeting Vector in Mammary Gland Cell 6. Expression of TMP Fc in Pichia pastoris and Identification of Its Biological Activity 7. Cloning and Expression of hTRF1 in Escherichia coli and Preparation of Polyclonal Antibody 8. Expression of Δ6-fatty Acid Desaturase Gene from Mortierella alpina in Pichia pastoris 9. Identification of Genes that are Specifically/preferentially Expressed in Developing Cotton Fibers by mRNA Fluorescence Differential Display (FDD) 10. Construction of Combined Site-directed Random Mutation Libraries of Recombinant Human Lymphotoxin 11. Expression and Purification of Three Fusion Proteins Containing a Single B|cell Epitope (β5、β9 or β8) of Human Chorionic Gonadotropin β Subunit 12. Construction of cDNA Library from NPC Tissue and Screening of Antigenic Genes 13. Identification of an Auxin Response Factor-like Protein cDNA from Mango Cotyledon Section 14. Preparation of Alpha-toxin’s Protective Antigen of Clostridium perfringens Type A and Research for Its Primary Immunological Protective Function 15. Construction of Two Robust CHO Cell Lines Resistant to Apoptosis and Adapted to Protein-free Medium by Over|expression of Igf-1/Bcl-2 or Bcl-2/Cyclin E Genes 16. Mammalian Gene-transfer and Expression Efficiencies of Baculovirus BacV-CMV-EGFPA 17. Overexpression of Escherchia coli Phytase with High Specific Activity 18. Physical and Chemical Characters of Recombinant Human Nucleoside Diphosphate Kinase A 19. Interface Domain of Hepatitis E Virus Capsid Protein Homodimer 20. Cloning of Human β2-microglobulin Gene and Its High Expression in Escherichia coli 21. Participation of Ions and Solutes on the Thermostability of α-amylase 22. Oriented Immobilization of Human IgG by Protein A on Imaging Ellipsometry Biosensor 23. Addition of TCA Cycle Intermediates Enhances Pyruvate Production 24. Transgenic Maize Plants with Low Copy Number of Foreign Genes were Produced with Maize Ubi-1 Promoter 25. Fermentation, Purification and Identification of Recombinant RGD-Hirudin 26. Expressing of N Gene Encoding Nucleocapsid Protein of Vesicular Stomatitis Virus and Elementary Application in ELISA 27. Construction of Recombinant Fowlpox Virus Expressing Chicken IL-2 and Assay of Biologic Activity of the Product in vitro No.2 1. Ubiquitin-proteasome Pathway and Virus Infection 2. Advances in the Research of Genetic Engineering of Heavy Metal Resistance and Accumulation in Plants 3. Construction of Human MAGE-3 DNA Vaccine and Its Immune Effects Observed in vivo 4. Construction of a Secreting Prokaryotic Shuttle Expressing Plasmid pBCG-SP-HSP65 and Its Expression in Mycobacterial smegmatis 5. Non-fused Expression of HAb18GEF by Reducing Stability of Translational Initiation Region in mRNA 6. Optimization of the Expression of Human DNA Topoisomerase Ⅰ in Pichia pastoris 7. Construction and Expression of Single Chain Fv Gene Against Domain Ⅲ of Human VEGF Receptor Ⅱ 8. Construction, Expression and Bioactivity Characterization of Targeting Toxin DT-VEGF 9. Expression of the Human Soluble Interleukin 4 Receptor in Methylotropic Yeast 10. Molecular Cloning of Two Rhipicephalus haemaphysaloides haemaphysaloides Cathepsin L-like Cysteine Proteinase Gene 11. Isolation of 2,4-Dichlorophenol Degrading Bacterium Strain and Cloning and Expression of Its 2,4-dichlorophenol Hydroxylase Gene 12. Induction of Hairy Roots of Panax ginseng and Studies on Suitable Culture Condition of Ginseng Hairy Roots 13. Protoplast Culture and Plant Regeneration of the Methionine Resistant Variant of Astragalus melilotoides Pall 14. Recovery and Purification of Monoclonal Antibody Producted byContinuous Perfusion Culture Using Expended Bed Adsorption 15. Characterization and Gene Cloning of the Endoglucanase from Pseudoalteromonas sp. DY3 Strain 16. Study on the Reconstitution in vitro and Photochemical Activities of Phytochrome from the Synechocystis sp. PCC6803 17. Influence of Wall Polymer and Preparation Process on the Particle Size and Encapsulation of Hemoglobin Microcapsules 18. Studies on in vitro Capacitation of Goat Spermatozoa by Heparin Treatment 19. Purification and Characterization of Recombinant Human anti-HAV Monoclonal Antibody 20. Particulate Recombinant Hepatitis E Virus Capsid Protein and Its Antigenicity and Immunogenicity 21. Effects of bkdAB Interruption on Avermectin Biosynthesis 22. Expression of N Domain of Chromogranin A in Bacillus subtilisand Its Antifungal Activity 23. Measurement of Intracellular pH in Long-chain Dicarboxylic Acid-producing Yeast Candida tropicalis and Its Growth Activity 24. Study on the Repeated Batch Fermentation of Lipase by Immobilized Rhizopus 25. Immobilization and Stabilization of Papain on SiO2 Particles Containing Amine Groups 26. Gene Modification and High Prokaryotic Expression of Porcine Interferon Alpha-1 27. Xylitol Production from Corn Cob Hemicellulosic Hydrolysate by Candida sp. 28. The Mechanism of Stat3 Nuclear Import 29. Effects of Veratryl Alcohol and Tween 80 on Ligninase Production and Its Roles in Decolorization of Azo Dyes by White-rot Basidiomycete PM2 30. Extraction and HPLC Analysis of Alkaloids in Goldenseal No.3 1. The Reverse Genetics Systems for Human and Animal RNA Viruses 2. Progress on Molecular Biology of Δ6-fatty Acid Desaturases 3. Application of Bioinformatics in Researches of Industrial Biocatalysis 4. XBP-1 Interacts with Estrogen Receptor α(ERα) 5. Construction of Recombinant Fowlpox Virus Coexpressing gp120 of Chinese HIV-1 Strain and IL-18 and Its Immunogenicity in Mice 6. Soluble Expression and Target Study to Brain of Anti- TfR ScFv 7. Studies of the Expression, Purification, Renaturation and Biologic Activity of an Anti-CEA Immunotoxin 8. Functional Expression of DNA Binding Domain of Zif268 in Escherichia coli 9. Screening Serum Response Special Antibodies of U251 Cell Line from Surface Display Phage Antibody Library 10. The ht-PAm cDNA Knock-in the Goat Beta-casein Gene Locus 11. Establishment of Saussurea involucrata Hairy Roots Culture and Plantlet Regeneration 12. Expression of Human μ-opioid Receptor cDNA in CHO Cell 13. Expression and Purification of Heptad Repeat Region of the Mumps Virus F Protein and Analysis of Characteristics 14. Preparation and Characterization of HLA-A*0201 Monomer and Tetramer Loaded with HCMV Antigenic Peptide 15. Affection of Metallothionein-3 to dUTPase's Accommodating Cellular Toxicity of dUTP 16. Construction of Protease Resistant Mutein of Human CNTF and Its Expression in Pichia pastoris 17. Preliminary Proteome Analysis for Saccharomyces cerevisiae under Different Culturing Conditions 18. Metabolic Flux Analysis of L-valine Fermentation in Corynebacterium glutamicum 19. High-cell Density Cultivation of Recombinant Escherichia coli for Production of TRAIL by Using a 2-stage Feeding Strategy 20. Media Optimization for Exopolysaccharide by Pholiota squarrosa (Pers. ex Fr.) Quel. AS 5.245 on Submerged Fermentation 21. Thermo-alkali-stable Catalase from Thermoascus aurantiacus and Its Potential Use in Textile Bleaching Process 22. Thiocyanate Elution Measurement of Relative Affinity of Phage Antibodies 23. The Second Editorial Board of Chinese Journal of Biotechnology 24. Isolation of Thermophilic Bacteria Thermus sp. YBJ-1 and Cloning of Amylase Gene 25. Expression of the Infectious Bursal Disease Virus Polyprotein in Vero Cells Using Attenuated Salmonella typhimurium as Transgenic Carrier 26. Enhanced Ergosterol Production by Recombinant Saccharomyces cerevisiae 1190 Harboring Vitreoscilla Hemoglobin Gene (vgb) 27. Studies on the Cell Growth, Differentiation and Terpene Lactone Accumulation in Ginkgo biloba Cell Suspension Cultures 28. Hepatitis E Virus Capsid Protein Production by High Cell Density Culture of Recombinant Escherichia coli 29. High Efficiency of L-Glutamine Production by Coupling Genetic Engineered Bacterial Glutamine Synthetase with Yeast Alcoholic Fermentation System 30. Cloning, Expression of Human Keratinocyte Growth Factor and Its Purification and Identification 31. Improving the Nutritional Value of Plant Foods Through Transgenic Approaches 32. The Gene wxcA of Xanthomonas campestris pv.campestris 8004 Strain Involved in EPS Yield 33. RNase Ⅲ-prepared Short Interfering RNAs Induce Degradation of SARS-coronavirus mRNAs in Human Cells 34. The Cloning,High Level Expression in Escherichia coli of Human Beta-Defensin 3 and Its Antimicrobial Activity Analysis 35. Expression of GST-HAI-1 Fusion Protein and Development of Monoclonal Antibody Against Human Hepatocyte Growth Factor Activator Inhibitor 1 36. Growth Inhibitory Effects of Recombinant Granzyme B Containing Different N-terminal Translocating Peptides 37. Identification of a Lens-specific cis-acting Element within the Basal Promoter of the Human Lens Intrinsic Membrane Protein MP19 Gene(LIM2) 38. Induction of Protective Immunity in Rhesus Monkey by Inoculation with Recombinant Fusion Protein of Cholera Toxin B Subunit-Multivalent Epitopes of Plasmodium falciparum 39. Sequencing and Bioinformatic Analysis of NRDRiso cDNA 40. Expression of Truncated gE Gene of Pseudorabies Virus(PRV) and Primary Application in Differential Diagnosis of PRV Vaccination and Infection 41. Construction and Characterization of a Pseudorabies Virus TK-/gG- Mutant 42. Carboxyl Terminus Truncated HPV58 Virus L1 Protein Expressed with Baculovirus System and its Bioactivity 43. Research on TALF Expression in Escherichia coli 44. Modification and High Level Expression of Protective Antigen Fragment of Botulinum Neurotoxin Serotype A 45. Cloning and Analysis of cDNA Encoding Key Enzyme Gene(dxr) of the non-MVA Pathway in Taxus chinensis Cells 46. Expression and Purification of Thermostable α-Glucuronidase from Thermotoga maritime 47. Experimental Study of Porous TCP to Generate Tissue-engineered Long Bone 48. DMSO Arrested Hybridoma Cells for Enhanced Antibody Production 49. Compared D-amino Acid Oxidase Expression in Different Pichia pastoris Host Strains 50. Study on the Properties of a Novel Glycine Amino Peptidase from Actinomucor elegans 51. Purification and Immobilization of Chondroitinase from Aeromonas sobria YH 311 52. Two-dimensional Electrophoresis Analysis of Proteins from the Colleterial Gland of Silkworm (Bombyx mori L.) 53. Expression and Characterization of Protein Disulfide Isomerase-related Protein A 54. The Primary Study on the Detection of Sterigmatocystin by Biologic Enzyme Electrode Modified with the Multiwall Carbon Nanotubes 55. The Labeling of 3T3-L1 Preadipocyte Cells with Enhanced Green Fluorescent Protein 56. Asymmetric Somatic Hybridization Between Mixed Wheat and Psathyrostachys juncea 57. The Expression of Growth Hormone Release Factor Gene Carried with PLGA Microspheres in Muscle Tissue and its Effect on Mouse Growth 58. Cloning,Expression and Purification of the C-terminal Section of Murine Heat Shock Protein gp96 59. A Motif-based Scanning Approach for Prediction of Protein Phosphorylation 60. Cytokine Aptamers as Therapeutic and Diagnostic Agents
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